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41.
Arbuscular mycorrhizal fungi (AMF) and N2-fixing legumes can alter the community structure of grasses. However, the effect of AMF, N2-fixing legumes, and their interaction on the dynamics of prairie grass communities remains unclear. The aim of this study was to clarify the influence of two AMF (Glomus cubense and Glomus sp.) and two legumes (Medicago sativa and Dalea purpurea) on the competitive relationship between three native cool-season (Elymus canadensis, Elymus trachycaulus ssp. subsecundus, and Elymus lanceolatus ssp. lanceolatus) and two native warm-season species of grasses (Schizachyrium scoparium and Bouteloua gracilis). Results show that AMF and legumes altered the community structure of the grasses. G. cubense favoured the productivity of warm-season B. gracilis when growing with M. sativa. This might be related to a negative impact of G. cubense on the nitrogen-fixing activity of M. sativa and to a lower N-use efficiency of E. canadensis and E. lanceolatus ssp. lanceolatus under competition. This suggested an increased ability of B. gracilis to use the available N resource as affected by more competitive species, whereas Glomus sp. reduced the competitive ability of this grass when associated with M. sativa. The decrease in B. gracilis biomass was thus likely caused by enhancement of P uptake by M. sativa over this grass. Glomus sp. was beneficial to S. scoparium, another warm-season species, in the absence of legumes, and this may be attributed to improved P-use efficiency of this grass under competition with cool season-grasses. In contrast, AMF and legumes were not beneficial for the cool season grasses. G. cubense depressed the growth of E. trachycaulus ssp. subsecundus, and M. sativa decreased nutrient uptake by cool-season native grasses. This study shows that beneficial effect of the arbuscular mycorrhizal symbiosis on the coexistence of warm-season grasses with more competitive cool-season grasses depends on the identity of the AMF symbiont, the presence of legume species, and nitrogen resource availability that was affected by the most competitive species or P-use efficiency of warm season species. 相似文献
42.
Water eutrophication and poultry manure require an efficacious remediation technology to decrease phosphorous (P) concentration. In this study, the P phytoremediation potential of a mining (ME) and nonmining ecotype (NME) of Pilea sinofasciata were analyzed by pot experiments employing different concentrations of P treatments (CK, P100, P200, P400, P800 and P1600) in tested soil. Growth, P accumulation and P-relevant enzyme activities of P. sinofasciata in ME and NME were assessed after 10 weeks’ plantation. The results showed that there was no significant difference between the shoot biomass of the ME and NME. Higher P concentration and accumulation were observed in the ME than NME for all treatments. Stem P concentration of the ME were 117%, 189%, 152%, 153%, 140% and 139% higher than the NME, and leaf P concentration were 122%, 146%, 138%, 144%, 137% and 113%. Maximum P uptake and phytoextraction was observed at P400 for the ME of P. sinofasciata. Elevated activities of acid phosphatase and phytase in roots were examined in P-enriched soil, and the ME presented higher activities compared with the NME. The results in this paper provide a theoretical basis for the use of ME of P. sinofasciata for phosphorus accumulators and suggest that ME of P. sinofasciata is a promising species for the excess P phytoextraction of P-enriched soils. 相似文献
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44.
Jie Kuai Xiaoyong Li Zhen Li Yan Xie Bo Wang Guangsheng Zhou 《Journal of Agronomy and Crop Science》2020,206(6):823-836
Waterlogging is a main factor causing rapeseed (Brassica napus L.) yield loss, and reasonable nitrogen (N) applications can compensate for this loss. To investigate the effects of N rates on seed yield of waterlogged rapeseed, the waterlogging-tolerant rapeseed variety ZS 9 and sensitive variety GH01 were waterlogged for 0 and 10 days with five leaves at the seedling stage under four N rates (0, 90, 180 and 270 kg/ha). Waterlogging significantly decreased seed yield, while N application can alleviate the yield loss. The yield decrease rate of waterlogged GH01 was greater than that of ZS 9 under the same N rate. During the seedling and bolting stage, the leaf photosynthetic rate (Pn) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity increased, while activities of adenosine diphosphate glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS) decreased with more N under the same watering conditions. Compared to the plants without waterlogging, the leaf Pn and Rubisco activity, starch and sucrose contents of waterlogged rapeseed decreased at the two stages; activities of AGPase, SuSy and SPS of waterlogged rapeseed decreased at the seedling while increased at the bolting stage for both the two varieties. At the flowering stage, the Pn, the activities of Rubisco, AGPase, SuSy, SPS and contents of sucrose, starch increased with more N application for both ZS 9 and GH01. Compared to the plants without waterlogging, the Pn and Rubisco activity for waterlogged plants of the two varieties increased; the waterlogged plants of tolerant variety had higher activities of AGPase, SuSy and SPS, while those of sensitive variety was significantly lower. However, the decreased starch and sucrose content were found in both tolerant and sensitive varieties. The activities of AGPase, SuSy and SPS at flowering were highly positively correlated with yield under the interactive effects of N and waterlogging. These results suggested that the flowering stage is the most important stage that N had the positive regulation on waterlogged rapeseed growth. The carbohydrates translocation from leaves to seeds of the tolerant variety were enhanced after waterlogging, while that of the sensitive variety was still inhibited. This was the main reason for the difference in yield between the two waterlogged varieties. 相似文献
45.
氮肥减量后移对喷灌玉米产量和水氮利用效率的影响 总被引:1,自引:0,他引:1
【目的】优化井灌区田间水肥管理。【方法】试验于2018年6—9月在河南省许昌灌溉试验站进行,以当地主栽玉米品种登海3737(P1)和豫单9953(P2)为试验材料,设置3种施肥调控方式,分别为当地传统施肥模式CK(N、P2O5、K2O施量分别为315、75、75 kg/hm^2,全部基施),优化模式F1(N、P2O5、K2O施量分别为225、75、75kg/hm^2,40%三叶期和60%拔节期追肥),优化模式F2(N、P2O5、K2O施量分别为225、75、75kg/hm^2,30%三叶期、30%拔节期和40%大喇叭口期追肥),研究了喷灌水肥一体化下氮肥减量后移对不同品种夏玉米生长发育、产量和水分利用效率的影响。【结果】增加施肥频次和施肥时间后移可提高玉米叶面积系数(LAI)和延缓叶片衰老,增加玉米干物质累积量以及最大生长速率。喷灌水肥一体化(F1、F2处理均值)较传统施肥籽粒产量提高7.8%,耗水量降低11.9%,水分利用效率(WUE)提高22.2%,籽粒氮肥偏生产力(PFPY)提高51.1%,生物量氮肥偏生产力提高49.2%。登海3737干物质累积、最大生长速率、WUE和PFPY的均值较豫单9953分别增加2.8%、7.7%、8.5%和8.6%,最大生长速率出现的时间没有差异。豫单9953干物质积累快增期持续时间比登海3737增加5.3 d。不同品种之间产量和构成要素差异极显著。登海3737平均产量为11 319 kg/hm^2,较豫单9953增产8.4%,其中穗长、百粒质量对产量贡献较大,分别提高22.5%和18.2%。【结论】本研究中,F2处理为最佳施肥模式,即N、P2O5、K2O施量分别为225、75、75 kg/hm^2,施肥配比为30%三叶期、30%拔节期、40%大喇叭口期。 相似文献
46.
Emiru Birhane Mengsteab Hailemariam Girmay Gebresamuel Tesfay Araya Kiros Meles Hadgu Lindsey Norgrove 《林业研究》2020,31(1):313-323
Poor land use management and practice inhibit the growth and establishment of tree seedlings in dryland areas.We assessed arbuscular mycorrhizal fungi(AM)status of Faidherbia albida(Del.)A.Chev.trees grown on different land uses.We quantified the growth and nutrient uptake of F.albida seedlings inoculated with AM from different sources.These efforts were based on soil and fine root samples from the rhizosphere soils of F.albida trees.AM root colonization was determined using the gridline intersect method.Spores were extracted by the wet sieving and decanting method and identified to genus level.The seedling experiment had a completely randomized onefactorial design with four treatments and five replications.Faidherbida albida seedlings were grown in a greenhouse.All in situ F.albida trees were colonized by AM fungi.AM root colonization of F.albida trees was significantly higher(P<0.0086)in area exclosures than on lands used for grazing or cultivation.Spore abundance was significantly higher(P<0.0014)in area exclosures followed by cultivated land and grazing land.Glomus was the dominant genus in all land-uses.AM-inoculated F.albida seedlings grew better(P<0.05)than non-inoculated controls.Seedlings inoculated with AM from area exclosure had significantly(P<0.05)higher growth and nutrient uptake than those inoculated with AM from grazing and cultivated land.This emphasizes the importance of the native soil AM potential for better establishment of seedlings to achieve optimum plant growth improvement and assist in rehabilitation of degraded arid lands. 相似文献
47.
为研究microRNA-124-3p(miR-124-3p)对H1N1亚型猪流感病毒(swine influenza virus,SIV)感染小鼠所致肺损伤的调控作用,本试验构建miR-124-3p腺病毒表达载体,通过小鼠尾部静脉注射法构建miR-124-3p差异表达小鼠模型,试验分3组:过表达组、抑制组和对照组。48 h后,各组小鼠鼻腔接种H1N1亚型SIV,每只105 EID50(50 μL)。连续观察14 d,计算小鼠平均体重变化率、观察病理切片并测定相关炎症因子IL-1β、TNF-α和IL-6 mRNA相对表达量。结果显示,已成功将pre-miR序列及其sponge序列插入腺病毒的穿梭质粒,并将其共转染293A细胞。实时荧光定量PCR检测证实,与对照组相比,过表达组和抑制组小鼠黑色素瘤细胞miR-124-3p表达水平分别极显著升高(P<0.01)和显著降低(P<0.05),表明成功构建腺病毒表达载体。过表达组、抑制组和对照组小鼠体重变化率分别为-5.5%、-12.4%和-8.6%。抑制组和对照组均可见肺泡壁增厚,其间有多量淋巴细胞浸润,部分肺泡内出现纤维蛋白渗出,且抑制组病理变化更为严重,肺泡中还有大量的红细胞浸润;而过表达组仅有少量的淋巴细胞浸润,肺脏组织较正常。与对照组相比,过表达组检测的炎症因子IL-1β、TNF-α和IL-6 mRNA表达水平均显著降低(P<0.05);抑制组炎症相关炎症因子mRNA表达水平均显著升高(P<0.05)。本试验结果表明,miR-124-3p对H1N1亚型SIV感染小鼠所致的肺脏炎症因子的表达具有抑制作用,同时能减轻肺脏病理损伤。 相似文献
48.
49.
A change in the European Union energy policy has markedly promoted the expansion of biogas production.Consequently,large amounts of nutrient-rich residues are being used as organic fertilizers.In this study,a pot experiment was conducted to simulate the high-risk situation of enhanced greenhouse gas (GHG) emissions following organic fertilizer application in energy maize cultivation.We hypothesized that cattle slurry application enhanced CO2 and N2O fluxes compared to biogas digestate because of the overall higher carbon (C) and nitrogen (N) input,and that higher levels of CO2 and N2O emissions could be expected by increasing soil organic C (SOC) and N contents.Biogas digestate and cattle slurry,at a rate of 150 kg NH4+-N ha-1,were incorporated into 3 soil types with low,medium,and high SOC contents (Cambisol,Mollic Gleysol,and Sapric Histosol,termed Clow,Cmedium,and Chigh,respectively).The GHG exchange (CO2,CH4,and N2O) was measured on 5 replicates over a period of 22 d using the closed chamber technique.The application of cattle slurry resulted in significantly higher CO2 and N2O fluxes compared to the application of biogas digestate.No differences were observed in CH4 exchange,which was close to zero for all treatments.Significantly higher CO2 emissions were observed in Chigh compared to the other two soil types,whereas the highest N2O emissions were observed in Cmedium.Thus,the results demonstrate the importance of soil type-adapted fertilization with respect to changing soil physical and environmental conditions. 相似文献
50.
不同温度下圆叶决明降解过程红壤可溶性氮及氮水解酶动态变化研究 总被引:1,自引:0,他引:1
为了探明圆叶决明(Chamaecrista rotundifolia)降解过程果园红壤供氮水平的变化规律,本研究采用模拟培养试验,研究15℃和25℃培养果园红壤硝态氮、铵态氮、可溶性总氮、可溶性有机氮含量的变化及脲酶、蛋白酶、天冬酰胺酶活性的变化。结果表明,圆叶决明降解过程果园红壤4种可溶性氮含量均显著提高,且在培养140 d达到最大值,25℃培养的效果更佳。其中,可溶性总氮、可溶性有机氮和硝态氮含量随时间的动态变化可用三次曲线方程来拟合。圆叶决明降解过程还能显著提高脲酶、蛋白酶和天冬酰胺酶的活性,其中蛋白酶活性和脲酶活性的变化可用指数方程和三次曲线方程来拟合。本研究认为圆叶决明降解过程可提高果园红壤可溶性氮含量,因此可通过翻压圆叶决明提高土壤的供氮水平。 相似文献